Overproduction of Hyperthermostable β-1,4-Endoglucanase from the Archaeon <i>Pyrococcus horikoshii</i> by Tobacco Chloroplast Engineering
Access this Article
Search this Article
One of the most cost-effective methods of producing industrial enzymes is by the use of transgenic plants. We demonstrated successful high-level expression of a hyperthermostable archaeal β-1,4-endoglucanase in mature tobacco leaves by transformation of chloroplasts by homologous recombination. The active recombinant enzyme was readily recovered not only from fresh but also from dried leaves.
- Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 77(10), 2140-2143, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry