Identification of Laboratory-Specific Variations of <i>Bacillus subtilis</i> Strains Used in Japan

Access this Article

Search this Article

Author(s)

Abstract

The strain of <i>Bacillus subtilis</i> 168 used in laboratories in Japan was distributed in the 1990s when the sequencing consortium commenced operations. After 20 years of use of <i>B. subtilis</i> 168 in many laboratories, observations of variations in growth phenotypes have been reported. In this study, to uncover laboratory-specific variations of <i>B. subtilis</i> 168 strains in Japan, we re-sequenced 11 <i>B. subtilis</i> 168 strains from nine laboratories and analyzed how their genomes differed. We found that the 168 strains from different laboratories differed by 1–7 variations. These variations might have been caused by differences in storage conditions in the laboratories or differences among colonies of the original stock. Based on our results, researchers ought to understand the genetic differences among wild-type (parental) strains in different laboratories and the reference strain by re-sequencing analysis, and ought to pay more attention to the management of laboratory strains.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 77(10), 2073-2076, 2013

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

Codes

  • NII Article ID (NAID)
    130003381893
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • ISSN
    0916-8451
  • NDL Article ID
    024972213
  • NDL Call No.
    Z53-G223
  • Data Source
    NDL  J-STAGE 
Page Top