Degradation of β-Conglycinin β-Homotrimer by Papain: Independent Occurrence of Limited and Extensive Proteolysis
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- SHUTOV Andrei D.
- Laboratory of Plant Biochemistry, Moldova State University
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- RUDAKOVA Angela S.
- Laboratory of Plant Biochemistry, Moldova State University
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- RUDAKOV Sergei V.
- Laboratory of Plant Biochemistry, Moldova State University
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- KAKHOVSKAYA Irina A.
- Laboratory of Plant Biochemistry, Moldova State University
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- SCHALLAU Anna A.
- Institute of Plant Genetics and Crop Plant Research (IPK)
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- WILSON Karl A.
- Department of Biological Sciences, State University of New York at Binghamton
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- MARUYAMA Nobuyuki
- Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
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抄録
Limited and extensive proteolysis occur when β-conglycinin β homo-trimer (β3-conglycinin) from soybeans is attacked by papain. Slow limited proteolysis is restricted to cleavage of β3-conglycinin polypeptides into subunit halves (N- and C-terminal domains) that are further slightly truncated. The kinetics of limited and extensive proteolyses analyzed separately indicates that the two processes occur independently from the very beginning of the reaction. In contrast, limited proteolysis of phaseolin from common beans has been found to be prerequisite for the onset of its extensive proteolysis. The dramatic distinction between the degradation patterns of β3-conglycinin and phaseolin, homologous storage 7S globulins, suggests the existence of intrinsic differences in their structures. This hypothesis is supported by comparative analysis of the accessibilities to the solvent of amino acid residues in phaseolin and β3-conglycinin structures, which indicated the relatively low packing density of the latter, resulting in enhanced susceptibility of it to extensive proteolysis.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (10), 2082-2086, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456854912
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- NII論文ID
- 130003381894
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC2c%2FjtFyjtQ%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024972224
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- PubMed
- 24096671
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可