The White-Rot Fungus <i>Pleurotus ostreatus</i> Transformant Overproduced Intracellular cAMP and Laccase

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  • YAO Yuki
    Environmental Science Graduate School, The University of Shiga Prefecture
  • SAKAMOTO Takaiku
    Environmental Science Graduate School, The University of Shiga Prefecture
  • HONDA Yoichi
    Graduate School of Agriculture, Kyoto University
  • KAGOTANI Yasuyuki
    Environmental Science Graduate School, The University of Shiga Prefecture
  • IZUMITSU Kousuke
    Environmental Science Graduate School, The University of Shiga Prefecture
  • SUZUKI Kazumi
    Environmental Science Graduate School, The University of Shiga Prefecture
  • IRIE Toshikazu
    Environmental Science Graduate School, The University of Shiga Prefecture

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  • The White-Rot Fungus Pleurotus ostreatus Transformant Overproduced Intracellular cAMP and Laccase

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Abstract

Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid.

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