A Ternary Conjugation System for the Construction of DNA Libraries for <i>Geobacillus kaustophilus</i> HTA426

Access this Article

Search this Article

Author(s)

    • WADA Keisuke WADA Keisuke
    • Applied Molecular Microbiology and Biomass Chemistry, Bioscience and Biotechnology, Graduate School, Kyushu University
    • DOI Katsumi
    • Microbial Genetic Division, Institute of Genetic Resources, Faculty of Agriculture, Graduate School, Kyushu University
    • OHSHIMA Toshihisa
    • Microbial Genetic Division, Institute of Genetic Resources, Faculty of Agriculture, Graduate School, Kyushu University

Abstract

This report describes efficient plasmid uptake by the thermophile <i>Geobacillus kaustophilus</i> HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for <i>G. kaustophilus</i> and to identify the genes for orotidine-5'-phosphate decarboxylase by <i>in vivo</i> functional screening. The results indicate that the conjugation system is useful in constructing <i>G. kaustophilus</i> libraries, which are practical in identifying thermophile genes.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 77(11), 2316-2318, 2013

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

Codes

  • NII Article ID (NAID)
    130003381932
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • ISSN
    0916-8451
  • NDL Article ID
    025061984
  • NDL Call No.
    Z53-G223
  • Data Source
    NDL  J-STAGE 
Page Top