A Ternary Conjugation System for the Construction of DNA Libraries for <i>Geobacillus kaustophilus</i> HTA426
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This report describes efficient plasmid uptake by the thermophile <i>Geobacillus kaustophilus</i> HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for <i>G. kaustophilus</i> and to identify the genes for orotidine-5'-phosphate decarboxylase by <i>in vivo</i> functional screening. The results indicate that the conjugation system is useful in constructing <i>G. kaustophilus</i> libraries, which are practical in identifying thermophile genes.
- Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 77(11), 2316-2318, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry