Production and Degradation of N-Acylhomoserine Lactone Quorum Sensing Signal Molecules in Bacteria Isolated from Activated Sludge

  • OCHIAI Seiji
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University JST, CREST
  • MOROHOSHI Tomohiro
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University JST, CREST
  • KURABEISHI Ayane
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University
  • SHINOZAKI Masahiro
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University JST, CREST
  • FUJITA Haruka
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University
  • SAWADA Isao
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University JST, CREST
  • IKEDA Tsukasa
    Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University JST, CREST

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タイトル別名
  • Production and Degradation of <i>N</i>-Acylhomoserine Lactone Quorum Sensing Signal Molecules in Bacteria Isolated from Activated Sludge

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N-Acylhomoserine lactones (AHLs) function as quorum-sensing signaling molecules in many Gram-negative bacteria. We isolated a total of 672 bacterial strains from activated sludge obtained from seven sewage treatment plants in Tochigi Prefecture, Japan, and screened for AHL-producing and degrading strains. Isolates (n=107) stimulated AHL-mediated purple pigment production in AHL reporter strains Chromobacterium violaceum CV026 and VIR07. Based on their 16S rRNA gene sequences, most of these AHL-producing isolates were assigned to the genus Aeromonas, and they were divided into six groups. Isolates (n=46) degraded N-decanoyl-L-homoserine lactone (C10-HSL) within 24 h. Based on their 16S rRNA gene sequences, the most dominant AHL-degrading isolates were assigned to the genus Acinetobacter and divided into six groups. Strains Ooi24, Omo91, and Uzu81, which showed higher C10-HSL-degrading activity, showed putative AHL-acylase activity.

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