Scavenging Systems for Reactive Carbonyls in the Cyanobacterium Synechocystis sp. PCC 6803

• SHIMAKAWA Ginga

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University

• SUZUKI Mayumi

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University

• YAMAMOTO Eriko

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University

• NISHI Akiko

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University

• SAITO Ryota

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University

• SAKAMOTO Katsuhiko

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University

• YAMAMOTO Hiroshi

  Department of Botany, Graduate School of Science, Kyoto University

• MAKINO Amane

  Department of Agriculture, Graduate School of Agricultural Science, Tohoku University Core Research for Evolutional Science and Technology, Japan Science and Technology Agency

• MIYAKE Chikahiro

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University Core Research for Evolutional Science and Technology, Japan Science and Technology Agency

書誌事項

タイトル別名

• Scavenging Systems for Reactive Carbonyls in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803

抄録

To elucidate the scavenging systems of sugar- and lipid-derived reactive carbonyls (RCs) in the cyanobacterium Synechocystis sp. PCC 6803 (S. 6803), we selected proteins from S. 6803 based on amino-acid (AA) sequence similarities with proteins from Arabidopsis thaliana, and characterized the properties of the GST-fusion proteins expressed. Slr0942 catalyzed the aldo-keto reductase (AKR) reaction scavenging mainly sugar-derived RCs, methylglyoxal (MG). Slr1192 is the medium-chain dehydrogenase/redutase (MDR). It catalyzed the AKR reaction scavenging several lipid-derived RCs, acrolein, propionaldehyde, and crotonaldehyde. Slr0315 is a short-chain dehydrogenase/redutase (SDR), and it catalyzed only the reduction of MG in the AKR reaction. Slr0381 catalyzed the conversion of hemithioacetal to S-lactoylglutahione (SLG) in the glyoxalase (GLX) 1 reaction. Sll1019 catalyzed the conversion of SLG to glutathione and lactate in the GLX2 reaction. GLX1 and GLX2 compose the glyoxalase system, which scavenges MG. These enzymes contribute to scavenging sugar- and lipid-derived RCs as scavenging systems.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (12), 2441-2448, 2013

  公益社団法人 日本農芸化学会

キーワード

• aldo-keto reductase (AKR)
• cyanobacteria
• medium-chain dehydrogenase/reductase (MDR)
• short-chain dehydrogenase/reductase (SDR)
• glyoxalase (GLX)

詳細情報

• CRID: 1390282681456959232
• NII論文ID: 130003381963
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.130554
• COI: 1:STN:280:DC%2BC2c3ks1altQ%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 025124374
• PubMed: 24317062
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025124374
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.130554
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可