Rapid degradation of poly(ADP-ribose) after injection into mouse bloodstream

• Okajima Yasuhisa

  Division of Genome Stability Research, National Cancer Center Research Institute

• Yoshida Tomoko

  ADP-ribosylation in Oncology Project, National Cancer Center Research Institute Kyoritsu University of Pharmacy Biochemistry Division, National Cancer Center Research Institute

• Fujimori Hiroaki

  Division of Genome Stability Research, National Cancer Center Research Institute

• Wang Junhui

  Division of Genome Stability Research, National Cancer Center Research Institute

• Harada Hiromi

  Division of Genome Stability Research, National Cancer Center Research Institute

• Suzuki Ylenia

  Biochemistry Division, National Cancer Center Research Institute Section of Biochemistry, Department of Life and Reproduction Sciences, University of Verona Strada Le Grazie

• Suzuki Hisanori

  Section of Biochemistry, Department of Life and Reproduction Sciences, University of Verona Strada Le Grazie

• Masutani Mitsuko

  Division of Genome Stability Research, National Cancer Center Research Institute Biochemistry Division, National Cancer Center Research Institute ADP-ribosylation in Oncology Project, National Cancer Center Research Institute

書誌事項

タイトル別名

• Rapid Degradation of Poly(ADP-ribose) after Injection into the Mouse Bloodstream

抄録

Extensive DNA damage leads to the activation of poly(ADP-ribose) polymerase and subsequently to the formation of poly(ADP-ribose). When the damage is severe or leads to cell death, poly(ADP-ribose) may leak into the blood circulation. The metabolism of poly(ADP-ribose) in the bloodstream is not well understood. Thus, in the present study, the metabolism of ³²P-labeled poly(ADP-ribose) was followed in mice after injection of this labeled compound into the tail vein. The results showed that 5 min after injection more than half of the radioactivity was concentrated in acid-soluble fractions, namely in low molecular weight compounds in the blood, liver, and kidneys. Most of this radioactivity was in the form of inorganic phosphate, detected 5 min post-injection in the blood, kidneys, and urine. By contrast, the metabolites ADP-ribose and phosphoribosyl-AMP were not detected in any of the tissues nor in blood or urine. Taken together, these findings suggest that once poly(ADP-ribose) enters the bloodstream it is rapidly degraded, thereby preventing its accumulation in the blood.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 36 (3), 462-466, 2013

  公益社団法人 日本薬学会

キーワード

• poly(ADP-ribose)
• poly(ADP-ribose) polymerase
• blood
• urine
• mouse

詳細情報

• CRID: 1390282679608787456
• NII論文ID: 130003382078
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b12-00753
• COI: 1:STN:280:DC%2BC3svit1ygtg%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 024284063
• PubMed: 23449331
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024284063
• Web Site: https://www.jstage.jst.go.jp/article/bpb/36/3/36_b12-00753/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013264995
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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