<i>In Vitro</i> Evaluation of Inhibitory Effect of Nuclear Factor-KappaB Activity by Small Interfering RNA on Pro-tumor Characteristics of M2-Like Macrophages
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- Kono Yusuke
- Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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- Kawakami Shigeru
- Graduate School of Biomedical Sciences, Nagasaki University
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- Higuchi Yuriko
- Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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- Yamashita Fumiyoshi
- Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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- Hashida Mitsuru
- Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University
Bibliographic Information
- Other Title
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- In Vitro Evaluation of Inhibitory Effect of Nuclear Factor-KappaB Activity by Small Interfering RNA on Pro-tumor Characteristics of M2-Like Macrophages
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Abstract
Tumor-associated macrophages (TAMs) have an alternatively activated macrophage phenotype (M2) and promote cancer cell proliferation, angiogenesis and metastasis. Nuclear factor-kappaB (NF-κB) is one of the master regulators of macrophage polarization. Here, we investigated the effect of inhibition of NF-κB activity by small interfering RNA (siRNA) on the pro-tumor response of macrophages located in the tumor microenvironment in vitro. We used mouse peritoneal macrophages cultured in conditioned medium from colon-26 cancer cells as an in vitro TAM model (M2-like macrophages). Transfection of NF-κB (p50) siRNA into M2-like macrophages resulted in a significant decrease in the secretion of interleukin (IL)-10 (a T helper 2 (Th2) cytokine) and a significant increase of T helper 1 (Th1) cytokine production (IL-12, tumor necrosis factor-α, and IL-6). Furthermore, vascular endothelial growth factor production and matrix metalloproteinase-9 mRNA expression in M2-like macrophages were suppressed by inhibition of NF-κB expression with NF-κB (p50) siRNA. In addition, there was a reduction of arginase mRNA expression and increase in nitric oxide production. The cytokine secretion profiles of macrophages cultured in conditioned medium from either B16BL6 or PAN-02 cancer cells were also converted from M2 to classically activated (M1) macrophages by transfection of NF-κB (p50) siRNA. These results suggest that inhibition of NF-κB activity in M2-like macrophages alters their phenotype toward M1.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 37 (1), 137-144, 2014
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204633660160
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- NII Article ID
- 130003382116
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- NII Book ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2c%2Fot1Ojuw%3D%3D
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- ISSN
- 13475215
- 09186158
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- HANDLE
- 10069/34195
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- NDL BIB ID
- 025136654
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- PubMed
- 24141263
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed