Biological Activity of Recombinant Bovine Interferon τ Produced by a Silkworm-Baculovirus Gene Expression System

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  • TAKAHASHI Hitomi
    Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305–0901, Japan
  • TSUNAZAKI Makoto
    Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305–0901, Japan Ehime Research Institute of Agriculture, Forestry an Fisheries, 7–156 Ashimo, Nomura, Iyo, Ehime 797–1211, Japan
  • HAMANO Takashi
    Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305–0901, Japan Livestock Technology Promotion Center, 695 Yamamoto, Hino, Gamou, Shiga 529–1651, Japan
  • TAKAHASHI Masashi
    Livestock and Grassland Research Division, NARO Kyushu Okinawa Agricultural Research Center, Suya 2421, Koshi, Kumamoto 861–1192, Japan
  • OKUDA Kiyoshi
    Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, 3–1–1, Tsushimanaka, Okayama, Okayama 700–8530, Japan
  • INUMARU Shigeki
    National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki 305–0856, Japan
  • OKANO Akira
    Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305–0901, Japan
  • GESHI Masaya
    Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305–0901, Japan
  • HIRAKO Makoto
    Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305–0901, Japan

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Abstract

Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to suppress PGF2α production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same bioactivity as native IFNτ.

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