Possible Involvement of HSP90-HSF1 Multichaperone Complex in Impairment of HSP72 Induction in the Failing Heart Following Myocardial Infarction in Rats

• Marunouchi Tetsuro

  Department of Molecular and Cellular Pharmacology, Tokyo University of Pharmacy and Life Sciences, Japan

• Araki Masato

  Department of Molecular and Cellular Pharmacology, Tokyo University of Pharmacy and Life Sciences, Japan

• Murata Mao

  Department of Molecular and Cellular Pharmacology, Tokyo University of Pharmacy and Life Sciences, Japan

• Takagi Norio

  Department of Molecular and Cellular Pharmacology, Tokyo University of Pharmacy and Life Sciences, Japan

• Tanonaka Kouichi

  Department of Molecular and Cellular Pharmacology, Tokyo University of Pharmacy and Life Sciences, Japan

抄録

It is generally accepted that an increase in the myocardial level of heat-shock protein 72 (HSP72) protects viable cardiac tissue against myocardial infarction (MI)-induced stress. However, the induction of HSP72 after exposure to heat shock (HS) is blunted in the failing rat heart following MI. The mechanisms underlying this impairment in the HSP72 induction ability of the failing heart are not yet clearly defined. In the present study, we examined the involvement in heat-shock factor 1 (HSF1), a transcription factor of HSPs, in decreased ability for HSP72 induction in the failing rat heart following MI. In the failing heart, nuclear translocation of the HSF1 after exposure to hyperthermia was markedly reduced, whereas HSF1 in the cytosolic fraction and the HSP90 chaperone complex containing HSF1, a repressor of HSF1, were increased. Treatment with an HSP90 inhibitor, 17-allylamino-17-demethoxygel-danamycin, appeared to dissociate the interaction of HSF1 with HSP90, and then induced HSP72 in the failing heart after exposure to hyperthermia. These results suggest that an increase in the multichaperone complex, especially the HSF1-HSP90 interaction, associated with attenuation of HSF1 translocation into the nucleus, was involved in the impairment of HS-induced HSP72 induction in the failing heart following MI.

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 123 (4), 336-346, 2013

  公益社団法人 日本薬理学会

キーワード

• chronic heart failure
• heat-shock factor-1
• heat-shock protein 72
• heat-shock protein 90
• multichaperone complex

詳細情報

• CRID: 1390001205180899328
• NII論文ID: 130003382614
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.13109fp
• COI: 1:CAS:528:DC%2BC2cXktlOqsA%3D%3D
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 025109254
• PubMed: 24285117
• Web Site: http://id.ndl.go.jp/bib/025109254
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025109254
• Web Site: https://www.jstage.jst.go.jp/article/jphs/123/4/123_13109FP/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014281201
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可