Development of Highly Precise Cell Stretching Microdevice and in-situ Observation of Stretched Cell

• Nakashima Yuta

  Graduate School of Science and Technology, Kumamoto University Graduate School of Science and Engineering, Yamaguchi University

• Monji Ryo

  Graduate School of Science and Engineering, Yamaguchi University

• Sato Katsuya

  Institute of Technology and Science, The University of Tokushima

• Minami Kazuyuki

  Graduate School of Science and Engineering, Yamaguchi University

Bibliographic Information

Other Title

• 高精度細胞伸展マイクロデバイスの開発と引張りを受ける細胞のその場観察
• コウセイド サイボウ シンテン マイクロデバイス ノ カイハツ ト ヒッパリ オ ウケル サイボウ ノ ソノ バ カンサツ

Abstract

This paper presents a cell stretching microdevice for in-situ observation of stretched cell. In a conventional cell stretching device, it was difficult to observe the initial and in-situ cellular response to stretching. The presented device consists of an elastic culture chamber and leaf springs by which uniaxial stretch is applied to the culture chamber. The device is produced by a micro three-dimensional structure fabrication process using multiple exposures to the photoresist. The device permits the observation of cellular responses to stretching in real time because it is made of transparent materials when observed by optical microscope. We demonstrated the fundamental characteristics of a microdevice, and observed the cellular response behavior by a fabricated microdevice. The amount of the culture chamber deformation was controlled by the amount of the stretching arm displacement. Also, we demonstrated the culture chamber during stretching was not deformed toward the optical axis. Moreover, in-situ observation of transition of the cellular calcium signaling response to stretching succeeded by optical microscopy using fabricated microdevice. These results indicated that this device can be used to observe and evaluate the initial cellular response and micro-strain field on a cell membrane during uniaxial stretching.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 133 (12), 350-357, 2013

  The Institute of Electrical Engineers of Japan

Keywords

• Cell stretching
• Mechanical stimulation
• Cellular response evaluation
• Cell biomechanics
• Microdevice

Details

• CRID: 1390282679439122816
• NII Article ID: 130003382695
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.133.350
• ISSN: 13475525
• ISSN: 13418939
• NDL BIB ID: 025090745
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025090745
• Web Site: https://www.jstage.jst.go.jp/article/ieejsmas/133/12/133_350/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed