Identification of the Source of <i>Francisella tularensis</i> Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis

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Author(s)

Abstract

Tularemia is a zoonotic disease caused by <i>Francisella tularensis</i>. Most patients in Japan have reportedly acquired such infections through direct contact with infected Japanese hares. We recently encountered a patient who contracted tularemia after skinning and butchering a dead hare. Because the remains of the hare were available, we attempted to determine whether the patient actually contracted infection by handling the carcass. <i>F. tularensis</i>-specific sequences were successfully amplified by PCR from the patient specimens as well as from the remnants of discarded hare carcass. PCR amplification of the IS<i>Ftu2</i> and RD1 regions indicated infection by <i>F. tularensis</i> subsp. <i>holarctica</i>, which was considered as a prevalent strain in Japan. Furthermore, high-resolution multiple-locus variable-number tandem repeat analysis (MLVA) showed that the combination of repeat numbers in sequences from the patient and hare were indistinguishable, thus indicating that the patient had been infected with <i>F. tularensis</i> strain that had also infected the hare. These findings demonstrated that MLVA is a useful epidemiological investigational tool to identify possible sources of certain zoonotic diseases such as tularemia.

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 66(6), 543-545, 2013

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

Codes

  • NII Article ID (NAID)
    130003390339
  • NII NACSIS-CAT ID (NCID)
    AA1132885X
  • Text Lang
    ENG
  • ISSN
    1344-6304
  • NDL Article ID
    025048832
  • NDL Call No.
    Z53-C450
  • Data Source
    NDL  J-STAGE 
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