In Vitro Growth and Maturation of Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles
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- HIRAO Yuji
- NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
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- SOMFAI Tamás
- NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
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- NARUSE Kenji
- NARO Tohoku Agricultural Research Center, Iwate 020-0198, Japan
書誌事項
- タイトル別名
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- <i>In Vitro</i> Growth and Maturation of Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles
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Cryopreservation of growing oocytes enriches the choice of timing and location of artificial embryo production. However, completion of oocyte growth after warming is crucial when using such cryopreserved oocytes. Our research objective was to develop a sequential system that incorporates cryopreservation of growing bovine oocytes and their subsequent in vitro growth. Oocyte-granulosa cell complexes with a mean oocyte diameter of approximately 100 µm were vitrified-warmed and then cultured for 14 days. The percentage of surviving oocytes following cryopreservation and 14-day culture was approximately 80%. More than half of the surviving oocytes were capable of maturing to metaphase II after in vitro maturation; the rate was comparable to that of control oocytes grown in vitro without cryopreservation. Taken together, the combined protocols for vitrification-warming of growing oocytes and subsequent in vitro growth can produce oocytes capable of undergoing meiotic maturation.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 60 (1), 68-72, 2014
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206337023872
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- NII論文ID
- 130003390814
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC2c%2FkvVGmtw%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 025304269
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- PubMed
- 24126072
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可