Delay in Cleavage of Porcine Embryos after Intracytoplasmic Sperm Injection (ICSI) Shows Poorer Embryonic Development

DOI Web Site Web Site PubMed 参考文献23件
  • NAKAI Michiko
    Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • OZAWA Manabu
    Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • MAEDOMARI Naoki
    Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan
  • NOGUCHI Junko
    Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • KANEKO Hiroyuki
    Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • ITO Junya
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan
  • ONISHI Akira
    Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
  • KASHIWAZAKI Naomi
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan
  • KIKUCHI Kazuhiro
    Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan

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In pigs, the embryonic developmental ability after intracytoplasmic sperm injection (ICSI) is inferior to that resulting from in vitro fertilization (IVF). We evaluated the timing of cell division up to blastocyst formation on embryonic development after ICSI using either whole sperm (w-ICSI) or the sperm head alone (h-ICSI) and IVF as a control. At 10 h after ICSI or IVF, we selected only zygotes, and each of the zygotes/embryos was evaluated for cleavage every 24 h until 168 h. We then observed a delay in the 1st and 2nd cleavages of h-ICSI embryos and also in blastocoele formation by w-ICSI embryos in comparison with IVF embryos. The rate of blastocyst formation and the quality of blastocysts in both ICSI groups were inferior to those in the IVF group. In conclusion, the delay in cleavage of porcine ICSI embryos shows poorer embryonic development.

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