cDNA cloning and characterization of chalcone isomerase-fold proteins from snapdragon (Antirrhinum majus L.) flowers

• Fujino Naoto

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Yamazaki Tatsuya

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Li Yanbing

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Kera Kota

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Furuhashi Erika

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Yamashita Satoshi

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Morita Yasumasa

  NARO Institute of Floricultural Science

• Nakayama Masayoshi

  NARO Institute of Floricultural Science

• Takahashi Seiji

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Nakayama Toru

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

書誌事項

タイトル別名

• cDNA cloning and characterization of chalcone isomerase-fold proteins from snapdragon (<i>Antirrhinum majus</i> L.) flowers

抄録

cDNAs coding for the chalcone isomerase-fold proteins (CIFP) of the snapdragon (Antirrhinum majus L.) were cloned and characterized. One of these CIFPs was a Cluster-1 member of the CIFP family, which was a catalytically active chalcone isomerase and thus termed AmCHI1. The recombinant AmCHI1 could act on 2′,4,4′,6′-tetrahydroxychalcone (THC) and 2′,3,4,4′,6′-pentahydroxychalcone (PHC) to produce naringenin and eriodictyol with k_cat/K_m values of 0.25 s⁻¹ µM⁻¹ and 0.071 s⁻¹ µM⁻¹, respectively, at pH 7.5 and 4°C. The enzyme could not act on 4′-O-glucosides of THC and PHC. In the yellow snapdragon petals (cv. Yellow Butterfly), the temporal expression patterns of AmCHI1 were consistent with the observed temporal accumulation patterns of flavones. Thus, regulation of the AmCHI1 transcription and substrate specificity of the expressed AmCHI1 should serve as the key mechanisms that allows for partitioning of the flavonoid biosynthetic pathways into the aurones and the non-aurone flavonoids in snapdragon petal cells. The other CIFP cDNA, AmCIFP4, was a Cluster-4 member of the family and was similar in its primary structure to enhancers of the flavonoid production of torenia (Torenia×hybrida) and petunia (Petunia×hybrida). AmCIFP4 was more abundantly expressed than AmCHI1 irrespective of flower color.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 31 (2), 105-114, 2014

  日本植物バイオテクノロジー学会

キーワード

• <i>Antirrhinum majus</i> L.
• aurone
• chalcone isomerase
• enhancer of flavonoid production
• snapdragon

詳細情報

• CRID: 1390282679305432832
• NII論文ID: 130003391376
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.14.0109c
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 025551000
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025551000
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可