Selection of Rodent Species Appropriate for mtDNA Transfer to Generate Transmitochondrial Mito-Mice Expressing Mitochondrial Respiration Defects Selection of Rodent Species Appropriate for mtDNA Transfer to Generate Transmitochondrial Mito-Mice Expressing Mitochondrial Respiration Defects

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Author(s)

    • ENOKI Shunkei Enoki Shunkei
    • Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan
    • Hayashi Chisato
    • Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan
    • Imanishi Hirotake
    • Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan|Japan Society for the Promotion of Science (JSPS), 8 Ichiban-cho, Chiyoda-ku, Tokyo 102-8472, Japan
    • Hashizume Osamu
    • Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan
    • Mekada Kazuyuki
    • RIKEN BioResource Center, Koyadai 3-1-1, Tsukuba-shi, Ibaraki 305-0074, Japan
    • Suzuki Hitoshi
    • Laboratory of Ecology and Genetics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-0810, Japan
    • Hashimoto Tetsuo
    • Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan
    • Nakada Kazuto
    • Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan

Abstract

Previous reports have shown that transmitochondrial mito-mice with nuclear DNA from <i>Mus musculus</i> and mtDNA from <i>M. spretus</i> do not express respiration defects, whereas those with mtDNA from <i>Rattus norvegicus</i> cannot be generated from ES cybrids with mtDNA from <i>R. norvegicus</i> due to inducing significant respiration defects and resultant losing multipotency. Here, we isolated transmitochondrial cybrids with mtDNA from various rodent species classified between <i>M. spretus</i> and <i>R. norvegicus</i>, and compared the O<sub>2</sub> consumption rates. The results showed a strong negative correlation between phylogenetic distance and reduction of O<sub>2</sub> consumption rates, which would be due to the coevolution of nuclear and mitochondrial genomes and the resultant incompatibility between the nuclear genome from <i>M. musculus</i> and the mitochondrial genome from the other rodent species. These observations suggested that <i>M. caroli</i> was an appropriate mtDNA donor to generate transmitochondrial mito-mice with nuclear DNA from <i>M. musculus</i>. Then, we generated ES cybrids with <i>M. caroli</i> mtDNA, and found that these ES cybrids expressed respiration defects without losing multipotency and can be used to generate transmitochondrial mito-mice expressing mitochondrial disorders.

Journal

  • Experimental Animals

    Experimental Animals 63(1), 21-30, 2014

    Japanese Association for Laboratory Animal Science

Codes

  • NII Article ID (NAID)
    130003391604
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1341-1357
  • NDL Article ID
    025152690
  • NDL Call No.
    Z54-H752
  • Data Source
    NDL  IR  J-STAGE 
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