Generation and Analysis of Serine Protease Inhibitor Kazal Type 3-Cre Driver Mice

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Author(s)

    • SAKATA Kazuya Sakata Kazuya
    • Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan|Department of Gastroenterological Surgery, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan
    • Araki Kimi
    • Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan
    • Suzuki Chigure
    • Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan
    • Ida Satoshi
    • Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan|Department of Gastroenterological Surgery, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan
    • Hashimoto Daisuke
    • Department of Gastroenterological Surgery, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan
    • Wang Jung
    • Department of Pathophysiology, College of Basic Medical Sciences, Dalian Medical University, 9 West Section, South Road, Lü Shun, Dalian 116044, P.R. China
    • Uchiyama Yasuo
    • Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan
    • Baba Hideo
    • Department of Gastroenterological Surgery, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan

Abstract

Serine protease inhibitor Kazal type 1 (<i>SPINK1</i>; mouse homologue <i>Spink3</i>) was initially discovered as a trypsin-specific inhibitor in the pancreas. However, previous studies have suggested that <i>SPINK1/Spink3</i> is expressed in a wide range of normal tissues and tumors, although precise characterization of its gene expression has not been described in adulthood. To further analyze <i>Spink3</i> expression, we generated two mouse lines in which either <i>lacZ</i> or Cre recombinase genes were inserted into the <i>Spink3</i> locus by Cre-<i>loxP</i> technology. In <i>Spink3<sup>lacZ</sup></i> mice, β-galactosidase activity was found in acinar cells of the pancreas and kidney, as well as epithelial cells of the bronchus in the lung, but not in the gastrointestinal tract or liver. <i>Spink3<sup>cre</sup></i> knock-in mice were crossed with Rosa26 reporter (R26R) mice to monitor Spink3 promoter activity. In <i>Spink3<sup>cre</sup></i>;R26R mice, β-galactosidase activity was found in acinar cells of the pancreas, kidney, lung, and a small proportion of cells in the gastrointestinal tract and liver. These data suggest that Spink3 is widely expressed in endoderm-derived tissues, and that <i>Spink3<sup>cre</sup></i> knock-in mice are a useful tool for establishment of a conditional knockout mice to analyze Spink3 function not only in normal tissues, but also in tumors that express <i>SPINK1/Spink3</i>.

Journal

  • Experimental Animals

    Experimental Animals 63(1), 45-53, 2014

    Japanese Association for Laboratory Animal Science

Codes

  • NII Article ID (NAID)
    130003391605
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • ISSN
    1341-1357
  • NDL Article ID
    025152738
  • NDL Call No.
    Z54-H752
  • Data Source
    NDL  J-STAGE 
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