Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate Sodium-Induced Colitis

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Author(s)

    • OKAMURA Midori Okamura Midori
    • Anatomy and Embryology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, 1–1–1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • YOH Keigyou Yoh Keigyou
    • Department of Nephrology, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, 1–1–1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • OJIMA Masami [他] Ojima Masami
    • Anatomy and Embryology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, 1–1–1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • Morito Naoki
    • Department of Nephrology, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, 1–1–1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • Takahashi Satoru
    • Anatomy and Embryology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, 1–1–1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan|International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1–1–1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan|Life Science Center, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1–1–1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan|Laboratory Animal Resource Center (LARC), University of Tsukuba, 1–1–1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan

Abstract

Ulcerative colitis (UC) is an inflammatory bowel disease, and its pathogenesis includes genetic, environmental, and immunological factors, such as T helper cells and their secreted cytokines. T helper cells are classified as Th1, Th2, and Th17 cells. However, it is unclear which T helper cells are important in UC. Dextran sulfate sodium (DSS)-induced colitis is a commonly used model of UC. In this study, we induced DSS colitis in Th1 dominant (T-bet transgenic (Tg)) mice, Th2 dominant (GATA-3 Tg) mice, and Th17 dominant (RORγt Tg) mice to elucidate the roles of T helper cell in DSS colitis. The results showed that GATA-3 Tg mice developed the most severe DSS colitis compared with the other groups. GATA-3 Tg mice showed a significant decreased in weight from day 1 to day 7, and an increased high score for the disease activity index compared with the other groups. Furthermore, GATA-3 Tg mice developed many ulcers in the colon, and many neutrophils and macrophages were detected on day 4 after DSS treatment. Measurement of GATA-3-induced cytokines demonstrated that IL-13 was highly expressed in the colon from DSS-induced GATA-3 Tg mice. In conclusion, GATA-3 overexpression in T-cells and IL-13 might play important roles in the development of DSS colitis.

Journal

  • Experimental Animals

    Experimental Animals 63(2), 133-140, 2014

    Japanese Association for Laboratory Animal Science

Codes

  • NII Article ID (NAID)
    130003391617
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • ISSN
    1341-1357
  • NDL Article ID
    025412016
  • NDL Call No.
    Z54-H752
  • Data Source
    NDL  J-STAGE 
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