エゾスナゴケ(<I>Racomitrium japonicum</I>)原糸体の低温長期保存法  [in Japanese] <I>In Vitro</I> Cryopreservation of Moss Protonema (<I>Racomitrium japonicum</I>)  [in Japanese]

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Author(s)

Abstract

エゾスナゴケ原糸体の無菌培養物を長期間簡易に維持する方法について検討した.エゾスナゴケの原糸体は,胞子嚢を表面殺菌することによって得た.原糸体培養物は,急激な凍結にも耐えた.また,前処理無しで-80 ℃に移しても,そのまま少なくとも1年間保存することが可能であり,再度培養を行うと生育を開始した.この結果から,エゾスナゴケの培養株を簡易に保存できることがわかった.

This study investigated the conditions for cryopreservation of the moss protonema (<I>Racomitrium japonicum</I>). An aseptic culture of <I>R. japonicum</I> was established from a single spore obtained from surface-sterilized sporangia. The cultured protonema showed extremely high tolerance to freezing. Even when directly transferred to -80°C without cryoprotectants, the cells remained viable one year later, and began growing shortly after direct transfer to fresh Knop medium maintained at room temperature. The growth and coloration of newly generated protonemata was affected by medium composition before and during long-term preservation. The suggested optimal medium for cryopreservation is Knop medium supplemented with sucrose (10g L<SUP>-1</SUP>) and trace elements (B, Mn, Zn, I, Mo, Cu and Co), with or without benzyladenine.

Journal

  • Shokubutsu Kankyo Kogaku

    Shokubutsu Kankyo Kogaku 26(1), 30-34, 2014

    Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists

Codes

  • NII Article ID (NAID)
    130003393023
  • NII NACSIS-CAT ID (NCID)
    AA12010914
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    1880-2028
  • NDL Article ID
    025317373
  • NDL Call No.
    Z18-3397
  • Data Source
    NDL  IR  J-STAGE 
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