Passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against Enterococcus faecalis
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- Kobayashi Yoshimi
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- Hayashi Makoto
- Department of Endodontics, Nihon University School of Dentistry
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- Yoshino Fumihiko
- Photomedical Dentistry, Department of Oral Science, Graduate School of Kanagawa Dental University
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- Tamura Muneaki
- Department of Microbiology, Nihon University School of Dentistry
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- Yoshida Ayaka
- Photomedical Dentistry, Department of Oral Science, Graduate School of Kanagawa Dental University
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- Ibi Haruna
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- Lee Masaichi-Chang-il
- Yokosuka-Shonan Disaster Health Emergency Research Center & ESR Laboratories, Graduate School of Kanagawa Dental University
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- Ochiai Kuniyasu
- Department of Microbiology, Nihon University School of Dentistry
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- Ogiso Bunnai
- Department of Endodontics, Nihon University School of Dentistry
書誌事項
- タイトル別名
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- Passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against <i>Enterococcus faecalis </i>
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Chemomechanical procedures can be used to eliminate bacteria from root canals. However, detectable bacteria sometimes remain because of the complexity of the root canal system. Endodontic passive ultrasonic irrigation (PUI) with hydrogen peroxide (H2O2) may be a promising option for increasing bactericidal hydroxyl radical (HO•) generation. In this in vitro experiment, we examined the effects of HO• generated using PUI and a low concentration of H2O2. An ultrasonic tip was submerged in 0.45 mol/L (1.5%) H2O2 in a microfuge tube. H2O2 was activated by an ultrasonic unit, the tip of which was kept centered in the tube, to mimic PUI. HO• generation was detected by electron spin resonance spectroscopy. An Enterococcus faecalis suspension in H2O2 was then preparedand activated as described above. Bactericidal effects were assessed by viable counting. Two-way analysis of variance and Tukey’s test were used to assess the statistical significance of differences among groups (P < 0.05). HO• generation and bactericidal activity were significantly increased by PUI in H2O2 in a time-dependent manner and were significantly higher than with H2O2 alone or with PUI in a Tris-HCl suspension. These results suggest that PUI in the presence of a low H2O2 concentration is a promising new disinfection strategy. (J Oral Sci 56, 35-39, 2014)
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
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Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 56 (1), 35-39, 2014
日本大学歯学部