Study on Measurement of Nanoparticles Size Based on Fluorescence Polarization (1st Report)

• ISHIZAKI Yuki

  大阪大学大学院

• HAYASHI Terutake

  大阪大学大学院

• MICHIHATA Masaki

  大阪大学大学院

• TAKAYA Yasuhiro

  大阪大学大学院

Bibliographic Information

Other Title

• 蛍光偏光法を用いたナノ粒子粒径計測に関する研究 (第1報)
• —Development of Rotational Diffusion Coefficient Measurement System Using Fluorescent DNA Probe—
• —蛍光DNAプローブを用いた回転拡散係数測定システムの開発—

Abstract

Nanoparticles are attractive materials in the area of nanotechnology, and there is a need to measure the particle size accurately and easily. We suggest a novel particle sizing method based on fluorescence polarization. Particle size evaluation can be achieved from measuring the rotational diffusion coefficient which is sensitive to the particle size. We develop the rotational diffusion coefficient measurement system using fluorescent DNA probe. We verify the feasibility of proposed method by a fundamental experiment. We measure the rotational diffusion coefficient of gold nanoparticle whose diameter is 8.2nm. The rotational diffusion coefficient decreases by 50% compared to the reference value in the measuring range. The result indicates that nanoparticle, whose diameter is as small as 8.2nm, can be measured.

Journal

• Journal of the Japan Society for Precision Engineering

  Journal of the Japan Society for Precision Engineering 80 (2), 214-219, 2014

  The Japan Society for Precision Engineering

Keywords

• nanoparticle
• particle sizing
• fluorescence polarization
• Brownian motion
• DNA

Details

• CRID: 1390282679805865344
• NII Article ID: 130003394534
• DOI: 10.2493/jjspe.80.214
• ISSN: 1882675X
• ISSN: 09120289
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed