Influence of thin carbonate-containing apatite coating with molecular precursor method to zirconia on osteoblast-like cell response
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- KANEKO Hajime
- Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College Department of Oral and Maxillofacial Implantology, Tokyo Dental College
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- SASAKI Hodaka
- Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College Department of Oral and Maxillofacial Implantology, Tokyo Dental College
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- HONMA Shinya
- Department of Oral and Maxillofacial Implantology, Tokyo Dental College
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- HAYAKAWA Tohru
- Department of Dental Engineering, Tsurumi University School of Dental Medicine
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- SATO Mitsunobu
- Coordination Engineering Laboratory, Faculty of Engineering, Kogakuin University
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- YAJIMA Yasutomo
- Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College Department of Oral and Maxillofacial Implantology, Tokyo Dental College
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- YOSHINARI Masao
- Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College
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Abstract
The influence of a thin carbonate-containing hydroxyapatite (CA) coating to tetragonal zirconia polycrystal (TZP) on osteoblastlike cell response was investigated. TZP disks were subjected to blasting and acid etching. Thin CA coatings were deposited by the molecular precursor method (TZP-CA). Initial cell adhesion of mouse osteoblast-like cells MC3T3-E1 was enhanced, and marked progress of actin filaments was observed on TZP-CA compared to on TZP. After 3, 5 or 7 days, cell proliferation on TZP-CA was significantly higher than that on TZP. Alkaline phosphatase activity was slightly lower on TZP-CA than on TZP at 7 days, and no difference was observed at 14 or 21 days. At 28 days incubation, collagenous fibers with mineral precipitants accompanied by phosphorous and amino groups were observed. These results indicate that thin CA coating with molecular precursor method offers promise as a means of enhancing cell response, particularly initial adhesion and proliferation of MC3T3-E1 cells.
Journal
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- Dental Materials Journal
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Dental Materials Journal 33 (1), 39-47, 2014
The Japanese Society for Dental Materials and Devices
