Evaluation of a Rapid Oral Bacteria Quantification System Using Dielectrophoresis and the Impedance Measurement
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- HIROTA KATSUHIKO
- Department of Oral Microbiology, Institute of Health Biosciences, The University of Tokushima Graduate School
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- INAGAKI SATORU
- Department of Microbiology, Tokyo Dental College
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- HAMADA RYO
- Incubation Center, Panasonic Healthcare Co., Ltd.
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- ISHIHARA KAZUYUKI
- Department of Microbiology, Tokyo Dental College
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- MIYAKE YOICHIRO
- Department of Oral Microbiology, Institute of Health Biosciences, The University of Tokushima Graduate School
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Abstract
To clarify the availability of the dielectrophoretic impedance measurement (DEPIM) system as the evaluator for oral care, we evaluated the usefulness of DEPIM system by comparison with the standard plate counting (SPC) method. First, the relationship between the DEPIM results and bacterial concentration measured by SPC was clarified. Next, the measurement of the microorganism number in a mixed suspension was evaluated with DEPIM and SPC. The bacterial counts with DEPIM strongly correlated with those with SPC (r2=0.633-0.997) and this correlation was also shown in the measurement of a mixed bacterial suspension (ranging from 105 to 108 cfu/ml) of two bacterial species. Moreover, the experiments using dissociating enzymes to eliminate the influence of the size of the bacterial aggregates demonstrated that the microbial measurement results with DEPIM are unaffected by bacterial aggregates. This study demonstrated that bacterial counts with DEPIM strongly correlated with those with SPC and were unaffected by bacterial aggregates.
Journal
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- Biocontrol Science
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Biocontrol Science 19 (1), 45-49, 2014
The Society for Antibacterial and Antifungal Agents, Japan