暗視野顕微鏡法におけるクラミドモナスの三次元トラッキング Three-Dimensional Tracking of <i>Chlamydomonas</i> in Dark Field Microscopy
Recently, flagella and cilia have gathered attension since their important role in mammal cells and relation to genetic diseases of human were revealed. <i>Chlamydomonas</i> is one of the model organism of flagella and phototaxis research. The purpose of our research is to track a freely swimming individual <i>Chlamydomonas</i> cell to observe its flagella and whole body movement with dark field microscopy to investigate the phototactic reaction of <i>Chlamydomonas</i>. In this paper, a method for three-dimensional tracking of <i>Chlamydomonas</i> using high-speed visual feedback in dark field microscopy is proposed. The method was implemented to an existing microscope tracking system. It demonstrated successful three-dimensional tracking of a swimimng <i>Chlamydomonas</i> for several tens of seconds. The precisions of the 3D position measurement were estimated as ±1[μm] (XY) and ±10[μm] (Z).
日本ロボット学会誌 31(10), 1028-1035, 2013