The supercritical CO₂ extract from the skin of <i>Bufo bufo gargarizans</i> Cantor blocks hepatitis B virus antigen secretion in HepG2.2.15 cells

• Cui Xiaoyan

  Translational Center for Stem Cell Research, Tongji Hospital, Department of Regenerative Medicine, Tongji University School of Medicine epato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo

• Inagaki Yoshinori

  epato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo

• Wang Dongliang

  epato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo

• Gao Jianjun

  epato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo

• Qi Fanghua

  epato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo

• Gao Bo

  Anhui China Resources Jinchan Pharmaceutical Co., Ltd.

• Kokudo Norihiro

  epato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo

• Fang Dingzhi

  Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University

• Tang Wei

  epato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University

Bibliographic Information

Other Title

• The supercritical CO2 extract from the skin of Bufo bufo gargarizans Cantor blocks hepatitis B virus antigen secretion in HepG2.2.15 cells

Abstract

The skin of Bufo bufo gargarizans Cantor has long been used for the treatment of hepatitis B in China and supercritical carbon dioxide extraction (SC-CO₂) is widely used in extracting active ingredients from natural products. The aim of present study was to assess the anti-hepatitis B virus (HBV) effect of the supercritical CO₂ extract from the skin of Bufo bufo gargarizans Cantor (SCE-BC). Cytotoxicity of SCE-BC was analyzed using an MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] assay in HepG2.2.15 cells. The hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and hepatitis B core-related antigen (HBcrAg) concentrations in cell culture medium were determined by chemiluminescent enzyme immunoassay. HBV mRNA in cells was determined using real-time polymerase chain reaction. SCE-BC concentrations below 10^-2 μg/mL had no significant toxicity to HepG2.2.15 cells. SCE-BC at 10^-4 μg/mL effectively inhibited the secretion of HBeAg by 23.36% on day 6. It was more potent than the positive control lamivudine (100 μg/mL) in terms of the inhibition of HBeAg and HBcrAg secretion on day 6. Consistent with the HBV antigen reduction, HBV mRNA expression was markedly inhibited in comparison to the control when HepG2.2.15 cells were treated with SCE-BC. Moreover, SCE-BC had greater inhibitory activity with respect to HBeAg than to HBsAg. Since HBeAg promotes immune tolerance and persistent infection during HBV infection, the present results suggest that immune tolerance induced by HBeAg might be overcome by SCE-BC. Therefore, SCE-BC warrants further investigation.

Journal

• BioScience Trends

  BioScience Trends 8 (1), 38-44, 2014

  International Research and Cooperation Association for Bio & Socio-Sciences Advancement

Keywords

• Hepatitis B virus
• traditional Chinese medicine
• Bufo bufo gargarizans Cantor
• HepG2.2.15 cells

Details

• CRID: 1390282680713412352
• NII Article ID: 130003397282
• DOI: 10.5582/bst.8.38
• ISSN: 18817823
• ISSN: 18817815
• PubMed: 24647111
• Web Site: https://search.jamas.or.jp/link/ui/2015097814
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed