Control of Arrangement for DNA Molecules Chemisorbed to the Organized Molecular Films of Comb Copolymers Containing <i>s</i>-Triazine

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  • Taguchi Makoto
    Department of Functional Materials Science, Faculty of Engineering, Saitama University
  • Fujimori Atsuhiro
    Graduate School of Science and Engineering, Saitama University

抄録

Adsorption behavior of DNA molecules to comb copolymers containing 2-vinyl-4, 6-diamino-1, 3, 5-triazine (VDAT) at air/water interface and their molecular arrangement have investigated by surface pressure-area (π-A) isotherm, IR spectroscopy, polarized UV-vis spectroscopy, and atomic force microscopy. A newly hydrogenated and fluorinated binary comb copolymers containing VDAT as adsorption template was synthesized by radical copolymerization. From the result of π-A isotherm of monolayer on DNA aqueous solution, an increase of value for molecular area per VDAT unit corresponds to 20 Å2. The IR spectrum of transferred LB multilayer indicates adsorption of DNA molecules by hydrogen bonding to the copolymer templates. AFM images show the circular domain at 50-60 nm diameters. Adsorbed DNA molecules showed the absorption band at 260 nm and emitted fluorescence at 400 nm. From the results of polarized UV-vis spectroscopy, these absorption bands indicate faint polarized dependency. In addition, emission bands in fluorescence spectrum of adsorbed DNA to copolymer film showed red-shift and became sharper than the one of their solution.

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