Detection of chemiluminescent labeling compounds by photographic method. Preliminary investigation on 1,10-phenanthroline-hydrogen peroxide and luminol-hydrogen peroxide systems.

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Author(s)

    • UENO Keihei
    • Department of Industrial Chemistry, Kumamoto Institute of Technology
    • SAGARA Fumio
    • Department of Industrial Chemistry, Kumamoto Institute of Technology
    • YOSHIDA Isao
    • Department of Industrial Chemistry, Kumamoto Institute of Technology
    • ENAMI Hiroko
    • Department of Industrial Chemistry, Kumamoto Institute of Technology
    • ETOH Shigeki
    • Department of Industrial Chemistry, Kumamoto Institute of Technology
    • SONODA Akinari
    • Department of Industrial Chemistry, Kumamoto Institute of Technology

Abstract

The following two chemiluminescent reaction systems were investigated for the photographic detection of ultra-micro amounts of analyte on an agarose gel plate. 1) Reaction of 1, 10-phenanthroline with hydrogen peroxide, which is catalyzed by copper(II) or osmium(VIII). 2) Reaction of luminol with hydrogen peroxide, which is catalyzed by iron(III) complex. Chemiluminescent light was detected using a high-speed instant photographic film, and amounts as small as 9×10<sup>-10</sup>mol of 1, 10-phenanthroline or 2×10<sup>-18</sup>mol of iron complex of tetraphenylporphinetrisulfonic acid were detected by this method.

Journal

  • Analytical Sciences

    Analytical Sciences 4(5), 477-480, 1988

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130003416700
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • Data Source
    J-STAGE 
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