Detection of chemiluminescent labeling compounds by photographic method. Preliminary investigation on 1,10-phenanthroline-hydrogen peroxide and luminol-hydrogen peroxide systems.
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The following two chemiluminescent reaction systems were investigated for the photographic detection of ultra-micro amounts of analyte on an agarose gel plate. 1) Reaction of 1, 10-phenanthroline with hydrogen peroxide, which is catalyzed by copper(II) or osmium(VIII). 2) Reaction of luminol with hydrogen peroxide, which is catalyzed by iron(III) complex. Chemiluminescent light was detected using a high-speed instant photographic film, and amounts as small as 9×10<sup>-10</sup>mol of 1, 10-phenanthroline or 2×10<sup>-18</sup>mol of iron complex of tetraphenylporphinetrisulfonic acid were detected by this method.
- Analytical Sciences
Analytical Sciences 4(5), 477-480, 1988
The Japan Society for Analytical Chemistry