Tachyplesins isolated from hemocytes of Southeast Asian horseshoe crabs (Carcinoscorpius rotundicauda and Tachypleus gigas): Identification of a new tachyplesin, tachyplesin III, and a processing intermediate of its precursor.
-
- Muta Tatsushi
- Department of Molecular Biology, Graduate School of Medical Science
-
- Fujimoto Tamiji
- Department of Biology, Faculty of Science, Kyushu University
-
- Nakajima Hiroshi
- Taiyo Central R & D Institute, Taiyo Fishery Co.,Ltd.
-
- Iwanaga Sadaaki
- Department of Molecular Biology, Graduate School of Medical Science
抄録
Tachyplesins and their analogs are antimicrobial peptides composed of 17 or 18 amino acid residues present abundantly in acid extracts of hemocyte debris of horseshoe crabs. We purified here tachyplesin isopeptides from hemocytes of two species of Southeast Asian horseshoe crabs, Carcinoscorpius rotundicauda and Tachypleus gigas, and determined their amino acid sequences. The major tachyplesin isolated from both species was identified, respectively, as tachyplesin I, which had previously been found in hemocytes of the Japanese horseshoe crab (Tachypleus tridentatus). The yield from both species was very high (more than 70 mg per 100 g wet weight of hemocytes), i.e., comparable with that from T. tridentatus. In addition to tachyplesin I, a new tachyplesin isopeptide, named tachyplesin III, was also isolated from T. gigas hemocytes, in which an arginine replaced the 15th lysine of tachyplesin I. The carboxyl-terminal residue of the isolated tachyplesins I and III was confirmed, respectively, to be an arginine α-amide by chemical analysis. Furthermore, a tachyplesin peptide derivative with a carboxyl-terminal extension of glycine-lysine was newly found in the hemocytes of C. rotundicauda. It appeared to be an intermediate derived from a tachyplesin precursor during processing to the mature form.
収録刊行物
-
- The Journal of Biochemistry
-
The Journal of Biochemistry 108 (2), 261-266, 1990
The Japanese Biochemical Society
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1572824502976268800
-
- NII論文ID
- 130003417337
-
- ISSN
- 0021924X
-
- 本文言語コード
- en
-
- データソース種別
-
- CiNii Articles