培養ヒト皮膚線維芽細胞の形態学的，免疫細胞化学的及び酵素細胞化学的検討　Ｆｉｂｒｏｈｉｓｔｉｏｃｙｔｏｉｄ　ｃｅｌｌ及び悪性線維性組織球腫との類似性について

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  • Morphological, immunocytochemical and enzyme-cytochemical studies of cultured human dermal fibroblasts. Similarities to the "Fibrohistiocytoid cell" and malignant fibrous histiocytoma.
  • similarities to the “Fibrohistiocytoid cell” and malignant fibrous histiocytoma
  • Fibrohistiocytoid cell及び悪性線維性組織球腫との類似性について

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We have recently reported that the existence of a specific cell type, called the “Fibrohistiocytoid (FH) cell”, which was found in a variety of chronic inflammatory tissues. This FH cell includes a series of cell types from metamorphosized fibroblast to a certain cell type which resembles a histiocytic fibroblast. In this study, to examine whether cultured human dermal fibroblast (FB) was transformed into a FH cell, it was studied using morphological, immunocytochemical and enzyme-cytochemical techniques. Three different population doubling level (PDL), 2-4 PDL, 15-20 PDL and 30-35 PDL FBs were examined. Morphologically, FBs had a short spindle shaped or elliptical cell body, an irregular shaped nucleus, fragmented rough endoplasmic reticula distributed in a dendritic pattern and a few dense bodies, especially marked in 15-20 PDL and 30-35 PDL FBs, but did not have monocyte/macrophage-specific characteristics of pseudopodia, multivesicular bodies and phagosomes. Cytochemically, all three different PDL FBs showed the positive reaction to α1-naphthyl butyrate esterase and acid phosphatase, and further only both of 15-20 PDL and 30-35 PDL FBs revealed the increasing immunoreactivity to α1-antitrypsin, α1-antichymotrypsin, lysozyme and ferritin. Moreover, the immunoreactivity to HLA-DR, HLA-DP and HLA-DQ was induced on 2-4 PDL and 15-20 PDL FBs treated with interferon-gamma. It has been considered that these immuno- and enzymecytochemical markers were found in FH cells as well as histiocytes in vivo, but never found in non-inflammatory fibroblasts in vivo. These data suggested that FBs under a certain condition in vitro expressed the analogous phenotype to FH cells. It seemed that FH cell might be a transformed fibroblast. Furtheremore, we also evaluated the similarity of FBs to MFH tumor cells in vivo and in vitro in the same way. MFH tumor cells revealed some similar phenotypes not only to FH cells but also FBs, suggesting that MFH might be originated from FH cells.

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