干潟底泥中の酵素活性の分布と変動―干潟生態系の分解機能を評価するための留意点―

• 広木 幹也

  国立環境研究所

• 花菱 香奈

  帝京大学理工学部

• 宇田川 弘勝

  日本学術振興会,国立環境研究所

• 矢部 徹

  国立環境研究所

• 佐竹 潔

  国立環境研究所

• 野原 精一

  国立環境研究所

書誌事項

タイトル別名

• Variations in enzyme activities in tidal flat sediments; considerations for evaluating their decomposing function in the ecosystem.
• 干潟生態系の分解機能を評価するための留意点

抄録

Since hydrolytic enzyme activities vary spatially and temporally in sediment, it is important to collect field samples using an appropriate method for the evaluation of their decomposing function in a tidal flat ecosystem. In this report, variations in enzyme activities were determined to establish the appropriate method for evaluating their ecological functions. A field survey was carried out in June and September of 2001, and to sediment samples were collected from 5 layers 0-75 mm deep at seven sites in the Banzu tidal flat located in the river mouth area of the Obitsu river. For each sample, three enzyme activities (β-glucosidase, GLU; β-acetylglucosaminidase, AGA; esterase, EST) were determined. Among all 70 samples, the mean and standard deviation values were, respectively, 0.022±0.012 unit g^-1 h^-1 (EST), 10.4±8.5μmol g^-1h^-1 (GLU), and 6.8±5.0μmol g^-1h^-1 (AGA). For testing the effects of sampling time, site location, and sampling layer depth on these enzyme activities, a three-way analysis of variance (ANOVA) was adopted, and showed that differences in site location, depth of the sampling layer and the interaction between those two parameters were significant. On the other hand, ANOVA results showed that among 56 subsurface samples (15-75mm) demonstrated that the difference in site location was significant, but that the difference in sampling layer depth had no significant effect on GLU or AGA activities while the interaction effect was not significant for any of the three enzyme activities. These results suggested that the sediment samples for measuring enzyme activities should be collected from subsurface layers to reduce the rate of sampling errors.

収録刊行物

• 陸水学雑誌

  陸水学雑誌 64 (3), 185-193, 2003

  日本陸水学会

キーワード

• アセチルグルコサミニダーゼ
• エステラーゼ
• グルコシダーゼ
• 酵素
• 盤洲干潟
• 分散分析法

詳細情報

• CRID: 1390282679036511232
• NII論文ID: 130003442259
• DOI: 10.3739/rikusui.64.185
• ISSN: 18824897
• ISSN: 00215104
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可