ＧＥＬＲＩＴＥ培地によるアンモニア酸化細菌の純粋分離

• 徳山 龍明

  日本大学農獣医学部農芸化学科

• 高橋 令二

  日本大学農獣医学部農芸化学科

書誌事項

タイトル別名

• On isolation of ammonia oxidizing bacteria from soil using gelrite solidified plates.

抄録

An enrichment culture of soil ammonia oxidizing bacteria was filtrated through a 2.0μm membrane filter (Nuclepore) to remove soil particles and filtrates were transferred to a fresh medium for the enrichment. An addition of antibiotics such as tetracycline and ampicillin and filtration through a 0.8μm membrane filter were useful to decrease contamitants. Using a higher enrichment culture colonies of ammonia oxidizing bacteria (300-600μm in diameter) were obtained on GELRITE-solidified plates. Cells picked up from the colony by means of capillary tubes were grown on a liquid media and produced nitrite from ammonia but did not show heterotrophic growth. Such isolation of ammonia oxidizing bacteria in culture using GELRITE-solidified plate was accomplished with comparative ease.

収録刊行物

• Bulletin of Japanese Society of Microbial Ecology（日本微生物生態学会報）

  Bulletin of Japanese Society of Microbial Ecology（日本微生物生態学会報） 4 (2), 101-105, 1989

  日本微生物生態学会・日本土壌微生物学会

キーワード

• Ammonia oxidizing bacteria
• Gelrite plate
• bacterial isolation

詳細情報

• CRID: 1390282679320626688
• NII論文ID: 130003442656
• DOI: 10.1264/microbes1986.4.101
• ISSN: 09117830
• Web Site: http://www.jstage.jst.go.jp/article/microbes1986/4/2/4_2_101/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可