Induction of protoplast-like cells in Bifidobacterium bifidum Es5. I. Induction of protoplasts and L-forms by penicillin.
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- NAKAI REIKO
- Department of Bacteriology, Tottori University School of Medicine
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- TAKAGI ATSUSHI
- Department of Bacteriology, Tottori University School of Medicine
書誌事項
- タイトル別名
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- I. INDUCTION OF PROTOPLASTS AND L-FORMS BY PENICILLIN
抄録
A method for protoplast induction with penicillin G (PCG) in Bifidobacterium bifidum Es5 was examined. Growing cells were converted into protoplasts in a nutrient broth containing PCG without addition of any osmotic stabilizers. The maximal rate of protoplast induction was approximately 50% after 6hr incubation with 1unit PCG per ml. Supplementing the PCG-treatment culture with 0.1 to 0.2M sucrose raised the induction rate to 80% or more. It is suggested that sucrose makes the bacteria more sensitive to PCG, and subsequently stabilizes the protoplasts. In addition, sucrose by itself caused a decrease in the number of viable cells but not in the turbidity of the culture without PCG.<br>L-colonies of strain Es5 developed from bacterial suspensions and also from protoplast suspensions inoculated on nutrient agar plates containing PCG alone or PCG and sucrose.
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 30 (3), 187-195, 1984
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
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詳細情報 詳細情報について
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- CRID
- 1390282679119884544
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- NII論文ID
- 130003458119
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- COI
- 1:CAS:528:DyaL2cXmtVOitr4%3D
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- ISSN
- 13498037
- 00221260
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可