ラットおよびウズラにおけるカドミウム結合蛋白質について

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  • Cadmium-Binding Protein in Tissues of Male Rats and Japanese Quail
  • ラット オヨビ ウズラ ニ オケル カドミウム ケツゴウ タンパクシツ ニ ツ

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Subcellular distribution of &lt109&gtCd and &lt109&gtCd incorporation into cadmium-binding protein (Cd-BP) were investigated in respective tissues of Cd-pretreated and intact control male rats and Japanese quail injected intravenously &lt109&gtCd-cadmium chloride. The liver contained the highest level of &lt109&gtCd of all the organs in the animals and birds examined. The largest amount of &lt109&gtCd was determined in the soluble cytoplasmic fraction derived from the homogenates of the liver, kidney and pancreas of control rats, as well as from those of the liver, kidney, pancreas and proventriculus of control quail. The crude nuclear fraction of the gastric gland of the control rats took up nearly half the amount of &lt109&gtCd contained in the homogenate of this gland of the control rats. Cd-exposure had in no case any significant effect either on the &lt109&gtCd uptake by the whole tissue or on the compartmentalization of &lt109&gtCd in the soluble fraction of any tissues in the rats and quail. In rats, as well as in quail the Sephadex gel filtration of the soluble fraction of each tissue gave rise to two peaks of &lt109&gtCd in the elution profile. The first peak was a minor one shown at the exclusion limit of Sephadex G-75 and the second peak a major one having an elution factor of approximately 2.0. The second peak of &lt109&gtCd was associated with a slight absorbance of ultraviolet (260nm) in the liver, but not in any other organ, of control rats and quail. Cd-exposure intensified both &lt109&gtCd peak and absorbance of ultraviolet. The second component of &lt109&gtCd was considered to be Cd-BP from its elution profile on Sephadex G-75. The Cd-BP-like component may be synthesized not only in the liver and kidney but also in the pancreas and gastric gland, regardless of the species of animal. Some possible mechanisms were discussed when Cd-BP had failed to prevent the initiation of Cd-toxicity.

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