動物体内におけるアミノグリコシッド系抗生物質の鑑別法
書誌事項
- タイトル別名
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- Microbiological and thin-layer chromatographic identification of aminoglycoside antibiotics in animal body.
- 動物体内におけるアミノグリコシッド系抗生物質の鑑別法〔英文〕
- ドウブツタイナイ ニ オケル アミノグリコシッドケイ コウセイ ブッシツ ノ
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Dihydrostreptomycin, kanamycin and fladiomycin were administered intramuscularly or orally to pigs and calves. An attempt was made to identify these aminoglycoside antibiotics qualitatively in tissues and urine collected from the animals after slaughter. The kidney, liver and muscle were homogenized and used to determine the diameters of inhibition zones to be produced on Micrococcus luteus ATCC 9341 and Bacillus subtilis ATCC 6633 plates at pH 6.0; and 8.0. The urine was used undiluted and after diluted serially. Thin-layer chromatographic identification was carried out only on kidney and urine samples characterized by capacity to produce larger inhibition zones on the B. subtilis plate at pH 8.0 than on any other plate. Next, the ultrafiltrate obtained from each tissue homogenate or urine was spotted on two silica gel plates. One of the Plates was developed in the upper layer of chloroform-methanol-17% ammonia (2:1:1), and the other in n-propanel-pyridine-acetic acid-water (15:10:3:12). The Rf values obtained were a little higher than those with reference antibiotic solutions in some cases. It was possible, however, to distinguish the three antibiotics contained in a kidney or urine sample from one another by thin-layer chromatography with two solvents. Thus, the methods mentioned above were considered to be available for the identification of aminoglycoside residues in slaughtered animals. When the kidney is suspected to contain some aminoglycosides, it is recommended to carry out chromatography on the extract from the renal cortex for the identification of these substances.
収録刊行物
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- 日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
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日本獸醫學雜誌(The Japanese Journal of Veterinary Science) 44 (2), 233-239, 1982
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681399176704
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- NII論文ID
- 110003947782
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- NII書誌ID
- AN00191788
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- COI
- 1:CAS:528:DyaL38XkslSrtbg%3D
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- ISSN
- 18811442
- 00215295
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- NDL書誌ID
- 2448731
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- PubMed
- 6180204
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
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- CiNii Articles
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- 使用不可