Isolation, characterization, and quantitative measurement of serum .ALPHA.1-acid glycoprotein in cattle.

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  • ウシα<SUB>1</SUB>酸性糖蛋白(α<SUB>1</SUB>-acid glycoprotein: α<SUB>1</SUB>AG)の分離精製, 性状及び定量について
  • ウシα1酸性糖蛋白(α1-acid glycoprotein α1 AG)の分離精製,性状及び定量について〔英文〕
  • ウシ アルファ 1 サンセイ トウ タンパク アルファ 1 acid glyc

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Abstract

Bovine (α1-acid glycoprotein (α1AG) was purified from pooled normal bovine sera by successive ammonium sulfate precipitation, ion-exchange chromatographies and gel filtration. Bovine α1AG had a molecular weight of 42, 000±2, 000 and a sedimentation coefficient of 3.4S. It contained 26.6% carbohydrate. Gel isoelectric focusing revealed a microheterogeneity with 7 to 8 bands in a pI range of 3.2 to 3.7. It migrated to the α1-globulin region upon immunoelectrophoresis. Single radial immunodiffusion was developed for the quantitative measurement of bovine α1AG in serum. The mean serum value of α1AG in 152 healthy Holstein cattle (1-12 years old) was 283.2±82.3pg/ml. Elevated values (cut-off value=450pg/ml) were observed in cattle with traumatic pericarditis (100%), arthritis (100%), mastitis (91%), pneumonia (70%), and mesenteric liponecrosis (43%).

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