Spontaneous Production of a C-Type RNA Virus in a Cell Line Derived from Rat Glioma

• IGARASHI Koichi

  Biological Research Laboratories, Central Research Division, Takeda Chemical Industries, Ltd.

• SASADA Reiko

• NIIYAMA Yasuhiko

• KOZAI Yoshio

• SUGINO Yukio

Abstract

The spontaneous production of a rat C-type RNA virus (ACV) in a cultured cell line (AC cells) established from a chemically induced rat glioma was studied. The characteristics of ACV were : morphology typical of C-type RNA virus; buoyant density of 1.15 g/ml in a sucrose density gradient; RNA directed DNA polymerase activity; viral core with a density of 1.28 to 1.30 g/ml ; 70S RNA with dimer structure; and structural protein composed of mainly four polypeptides. Kinetical analysis of DNA-DNA hybridization revealed that DNA sequences homologous to DNA transcripts of RNA of ACV were present in rat cells. RNA directed DNA polymerase of ACV partially cross-reacted with antiserum to the polymerase of Rauscher murine leukemia virus. These data suggest that ACV is an endogenous C-type RNA virus of rat origin.

Journal

• Japanese Journal of Microbiology

  Japanese Journal of Microbiology 23 (1), 1-15, 1979

  Center For Academic Publications Japan

Details

• CRID: 1570854178105003904
• NII Article ID: 130003482748
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00309234809?from=CiNii
• Text Lang: en
• Data Source:
  • CiNii Articles