Isolation and Characterization of a Filamentous Phage, Vf33, Specific for <i>Vibrio parahaemolyticus</i>

J-STAGE
  • TANIGUCHI Hatsumi
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • SATO Kiyoshi
    Onga Health Center
  • OGAWA Midori
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • UDOU Takezo
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • MIZUGUCHI Yasuo
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health

抄録

Phage Vf33, a filamentous phage about 1, 400nm long and 7nm wide, specific for Vibrio parahaemolyticus, was isolated and characterized. The buoyant density of Vf33 in CsCl was 1.292g/cm3. As with other filamentous phages, the lytic activity of Vf33 was resistant to heating below 80C and to treatment with diethylether, acetone or methanol but sensitive to chloroform. The nucleic acid of this phage is single-stranded circular DNA 8.4kb in size. The viral genome was converted to a double-stranded replicative form in the host cell. Among the strains tested, only V. parahaemolyticus strains possessing K38 antigen was sensitive to the phage.

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