Abortive Infection of L Cells by Influenza B Virus

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Author(s)

    • TAKEURA Shigeki
    • 1st Department of Internal Medicine, Nagoya University School of Medicine
    • AOKI Hiizu
    • Laboratory of Virology, Research Institute for Disease Mechanism and Control
    • TSURUMI Tatsuya
    • Laboratory of Virology, Research Institute for Disease Mechanism and Control
    • FUJIOKA Hisashi
    • Department of Medical Zoology, Nagoya University School of Medicine
    • MAENO Koichiro
    • Laboratory of Virology, Research Institute for Disease Mechanism and Control

Abstract

Host-dependent restriction of influenza B virus replication in L cells was analysed in comparison with productive infection in MDCK or 1-5C-4 cells. The synthesis and intracellular distribution of virus-specific proteins and the production of cytoplasmic ribonucleoproteins in nonpermissive L cells were similar to those in permissive MDCK cells. However, an electron microscopic study of infected L cells showed neither extracellular virions nor budding virus particles on the cell surface, in contrast to MDCK cells which produced numerous virus particles. PAGE analysis of the plasma membrane isolated from the cells demonstrated no significant difference in the composition of viral polypeptides between permissive 1-5C-4 and nonpermissive L cells. It was noted that the abortiveness of influenza B virus infection in L cells may be due to a defect in host cell function involved in the initiation of virus budding.

Journal

  • Japanese Journal of Microbiology

    Japanese Journal of Microbiology 28(4), 427-437, 1984

    Center For Academic Publications Japan

Codes

  • NII Article ID (NAID)
    130003483039
  • Text Lang
    EN
  • ISSN
    0385-5600
  • Data Source
    J-STAGE 
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