Solubilization and Partial Properties of Receptor Substance for Bacteriophage α2 Induced from <i>Clostridium botulinum</i> Type A 190L

J-STAGE
  • TAKUMI Kenji
    Department of Food Microbiology, Tokushima University School of Medicine
  • TAKEOKA Aya
    Department of Food Microbiology, Tokushima University School of Medicine
  • KINOUCHI Takemi
    Department of Food Microbiology, Tokushima University School of Medicine
  • KAWATA Tomio
    Department of Food Microbiology, Tokushima University School of Medicine

抄録

Bacteriophage α2, one of the two inducible phages from Clostridium botulinum type A 190L, had a latent period of 55min and an average burst size of 75 in C. botulinum type A Hall used as the host bacterium. The phage particles were adsorbed on the cell walls extracted with hot trichloroacetic acid (TCA-walls). The receptor substance for the phage was solubilized from the TCA-walls with Achromopeptidase and fractionated by gel filtration on Sephadex G-150. The fraction having the highest level of receptor activity for the phage contained large amounts of muramic acid and glucosamine. Both authentic muramic acid and glucosamine significantly inactivated the phage, whereas glucose, galactose, L-and D-alanine, diaminopimeric acid, or D-glutamic acid did not exhibit similar activity. There results strongly suggest that the receptor site for phage α2 is closely associated with glycan moieties of the cell wall peptidoglycan.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ