Human Macrophage-Activating Factors for Cytotoxicity

J-STAGE
  • HIGUCHI Masahiro
    Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo
  • SUGIMOTO Masamichi
    Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo
  • KOBAYASHI Yoshiro
    Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo
  • OSAWA Toshiaki
    Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo

抄録

Two different factors (MAF-C I and MAF-C II) were obtained by anion exchange chromatography of the culture supernatant of a human T-cell hybridoma, H3-E9-6, which produces macrophage-activating factors for cytotoxicity (MAF-C). These 2 factors induced the cytotoxicity of monocytes synergistically as a priming signal (MAF-C I) and a triggering signal (MAF-C II), respectively. On gel filtration on a column of Superose 12, MAF-C II was eluted mainly at the void volume, whereas MAF-C I was eluted in the fractions corresponding to approximate molecular weights of 30-300K. On the other hand, gel filtration in the presence of sodium deoxycholate revealed that MAF-C II has an approximate molecular weight of 40, 000, but MAF-C I was unstable under these conditions. When the activity for mouse macrophages (MAF-Cm activity) was tested, the MAF-C II fraction showed high MAF-Cm activity in the presence of murine recombinant interferon gamma (rIFN-γ), but the MAF-C I fraction did not show MAF-Cm activity even in the presence of lipopolysaccharide (LPS). These results suggest that MAF-C I (priming lymphokine) has species specificity but MAF-C II (triggering lymphokine) does not.

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