Simplified Method for Preparation of Concentrated Exoproteins Produced by <i>Staphylococcus aureus</i> Grown on Surface of Cellophane Bag Containing Liquid Medium
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- IKIGAI Hajime
- Department of Microbiology, The Jikei University School of Medicine
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- SEKI Keiko
- Department of Microbiology, The Jikei University School of Medicine
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- NISHIHARA Shoko
- Department of Microbiology, The Jikei University School of Medicine
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- MASUDA Shogo
- Department of Microbiology, The Jikei University School of Medicine
抄録
A simplified method for preparation of concentrated exoproteins including protein A and α-toxin produced by Staphylococcus aureus was successfully devised. The concentrated proteins were obtained by cultivating S. aureus organisms on the surface of a liquid medium-containing cellophane bag enclosed in a sterilized glass flask. With the same amount of medium, the total amount of proteins obtained by the method presented here was identical with that obtained by conventional liquid culture. The concentration of proteins obtained by the method, however, was high enough to observe their distinct bands stained on polyacrylamide gel electrophoresis. This method was considered quite useful not only for large-scale cultivation for the purification of staphylococcal proteins but also for small-scale study using the proteins. The precise description of the method was presented and its possible usefulness was discussed.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 32 (2), 225-228, 1988
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1571135653081839744
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- NII論文ID
- 130003483428
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles