Spreading of Streptomycin Antibiotic Resistance Gene in <i>Escherichia coli</i> Plasmids Demonstrated by Southern Blot Analysis

• HAJNAL Anna

  National Institute of Hematology and Blood Transfusion

• FALUS Andras

  National Institute of Rheumatology and Physiotherapy

抄録

Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous. However, the streptomycin (Sm) resistance genes of the plasmids were identical or closely homologous in 29 of the 33 plasmids conferring Sm resistance. These data were based on Southern blot analysis, using the Sm resistance gene (encoding aminoglycoside phosphoryl transferase) as probe cut out from pBP1 plasmid. Our data suggest an extensive spreading of streptomycin resistance gene of this type.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 33 (6), 441-447, 1989

  Center For Academic Publications Japan

詳細情報

• CRID: 1574231877825784320
• NII論文ID: 130003483576
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00173744824?from=CiNii
• 本文言語コード: en
• データソース種別:
  • CiNii Articles