Protection of Mice against Sendai Virus Pneumonia by Non-Neutralizing Anti-F Monoclonal Antibodies

J-STAGE
  • MOCHIZUKI Yasushi
    Department of Microbiology, Kobe University School of Medicine
  • MING Tan de
    Department of Microbiology, Kobe University School of Medicine
  • HAYASHI Takako
    Department of Microbiology, Kobe University School of Medicine
  • ITOH Masae
    Department of Microbiology, Kobe University School of Medicine
  • HOTTA Hak
    Department of Microbiology, Kobe University School of Medicine
  • HOMMA Morio
    Department of Microbiology, Kobe University School of Medicine

抄録

Nine monoclonal antibodies (MAbs) directed to F protein of Sendai virus were obtained and characterized for their protective ability against Sendai virus infection in mice. None of the MAbs showed hemagglutination-inhibition (HI), hemolysis-inhibition (HLI), or neutralization (NT) activities in vitro when assayed by standard methods. Some of the MAbs, however, showed complement-requiring NT (C-NT) and complement-requiring hemolysis (C-HL) activities when assayed in the presence of complement. Passive immunization experiments revealed that the MAbs with higher C-NT and C-HL activities showed protective activity against Sendai virus pneumonia in mice, and that some MAbs with IgG1 isotype having neither C-NT nor C-HL activity also showed the protective activity. Digestion of the MAbs with pepsin which split immunoglobulin molecules into F(ab')2 and Fc fragments greatly suppressed the protective activity. These results suggest that not only complement-mediated immunological responses such as immune virolysis but also antibody-dependent cellular cytotoxicity (ADCC) and/or immune phagocytosis, in which complement system is not necessarily involved, play an important role in the protection of mice from Sendai virus infection.

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