Serological Studies on <i>Leptospira</i> Strain Ictero No. I Using Monoclonal Antibodies
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- KOBAYASHI Yuzuru
- First Department of Internal Medicine, School of Medicine, Ehime University
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- SADA Eiji
- First Department of Internal Medicine, School of Medicine, Ehime University
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- TAMAI Tomonori
- First Department of Internal Medicine, School of Medicine, Ehime University
抄録
In order to elucidate the full serological characteristics of strain Ictero No. I, the first strain of Leptospira isolated by Inada and Ido in 1914, 17 monoclonal antibodies against the strain were produced by cell fusion technology. The antibody-producing hybridomas were designated IMAs 1 to 17. The reactivities of the monoclonal antibodies produced by the hybridomas were determined by the microscopic agglutination test. One of the 17 monoclonal antibodies, IMA 1, reacted with strains Ictero No. I, LT 1131 and Naam, but not with other strains of the serogroup Icterohaemorrhagiae including strain RGA used in the present study. Moreover, the reactivity of the antigenic determinant of IMA 1 was inactivated by treatment at 56C for 30min. The 16 other monoclonal antibodies, IMAs 2 to 17, showed different reaction patterns against Leptospira strains of the serogroup Icterohaemorrhagiae. All of the 16 antibodies reacted with both Ictero No. I and RGA strains. The antigens against antibodies IMAs 2 to 17 were thermostable. The present study thus clarified the presence of a thermolabile antigen in strain Ictero No. I, and allowed correct distinction between the serotype of strain Ictero No. I and that of strain RGA using IMA 1. Therefore, we propose that strain Ictero No. I represents serovar icterohaemorrhagiae, as originally designated by Inada and Ido. Moreover, strain Ictero No. I should be designated as the type strain of Leptospira interrogans.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 35 (1), 67-76, 1991
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1570291228150860032
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- NII論文ID
- 130003483709
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles