Serial Observations of Chronic Rotavirus Infection in an Immunodeficient Child

J-STAGE
  • OISHI Isao
    Laboratory of Virology, Osaka Prefectural Institute of Public Health
  • KIMURA Teruo
    Department of Epidemiology, Osaka City Institute of Public Health and Environmental Sciences
  • MURAKAMI Tsukasa
    Department of Epidemiology, Osaka City Institute of Public Health and Environmental Sciences
  • HARUKI Kosuke
    Department of Epidemiology, Osaka City Institute of Public Health and Environmental Sciences
  • YAMAZAKI Kenji
    Laboratory of Virology, Osaka Prefectural Institute of Public Health
  • SETO Yoshiyuki
    Department of Epidemiology, Osaka City Institute of Public Health and Environmental Sciences
  • MINEKAWA Yoshiichi
    Laboratory of Virology, Osaka Prefectural Institute of Public Health
  • FUNAMOTO Hitokazu
    Department of Pediatrics, Osaka City Momoyama General Hospital

抄録

Chronic rotavirus infection of an infant with severe combined immunodeficiency (SCID) was studied by virological examinations in association with long-term observation of his symptoms and immune status. During eleven months of hospitalization, the patient was suffering from incurable severe diarrhea with persisting excretion of rotaviruses detected by electron microscopy and the reversed-passive hemagglutination (R-PHA) test and had transient hepatitis symptom despite multiple administrations of human gammaglobulin and high calorie fluids. The detected viruses were morphologically recognized as rotavirus with double capsid structure. Polyacrylamide gel electrophoretic (PAGE) analysis of their genomic RNAs showed the long electropherotype of group A virus with abnormal migration profiles changing considerably from the early to the late phase of illness: (1) The 11th segment became undetectable; (2) the molecular weight of the 6th segment slightly increased; (3) seven to fourteen extra segments appeared; and (4) PAGE patterns of viral genomic RNAs changed every three or four months. These findings suggest that chronic infection with rotavirus accompanied the generation of extra viral genomic segments and their unusual assortments in an immunodeficient host.

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