Factors Influencing the <i>in vitro</i> Growth of <i>Mycobacterium</i> <i>leprae</i>
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- DHOPLE Arvind Madhav
- Department of Biological Sciences, Florida Institute of Technology
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- LAMOUREUX Laurie Christine
- Department of Biological Sciences, Florida Institute of Technology
抄録
In an attempt to determine the factors that influence the in vitro growth of Mycobacterium leprae in DH medium, the effects of sulfhydryl compounds were studied. Growth of M. leprae was monitored using two biochemical indicators. Only the sulfhydryl compounds, in reduced form, containing carboxyl group could support the growth of M. leprae. Higher cell yields were obtained when these sulfhydryl compounds were supplemented with dithiothreitol, presumable to keep the monothiols in reduced state during long incubation periods. Ascorbic acid could not replace dithiothreitol for this purpose. It is suggested that these carboxylated sulfhydryl compounds play a role in the metabolic activity of M. leprae along with maintaining low redox potential of the medium.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 35 (3), 209-213, 1991
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1573105977917970176
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- NII論文ID
- 130003483757
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles