A Unique Monoclonal Antibody That Recognizes Mature p17 of HIV-1 but Not Its Precursor

J-STAGE
  • SAITOH Atsushi
    Department of Experimental Chemotherapy, Research Institute for Microbial Diseases, Osaka University
  • TANAKA Naoto
    Department of Experimental Chemotherapy, Research Institute for Microbial Diseases, Osaka University
  • NAKATA Atsuo
    Department of Experimental Chemotherapy, Research Institute for Microbial Diseases, Osaka University
  • IKUTA Kazuyoshi
    Section of Serology, Institute of Immunological Science, Hokkaido University
  • SHINAGAWA Hideo
    Department of Experimental Chemotherapy, Research Institute for Microbial Diseases, Osaka University

Abstract

The entire and partial gag regions of human immunodeficiency virus type 1 (HIV-1) were overproduced in Escherichia coli and used for epitope mapping of antibodies against p17. We found that a mouse monoclonal antibody to p17, V17 recognizes the mature p17 but not the unprocessed Gag proteins containing the entire p17 moiety. Further analysis revealed that V17 recognizes the C-terminal 12-amino-acid region of p17 having free C-terminus. This monoclonal antibody may be useful for monitoring the maturation of virus particles.

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