Pili of <i>Vibrio cholerae</i> Widely Distributed in Serogroup O1 Strains

J-STAGE
  • Iwanaga Masaaki
    Department of Bacteriology, University of the Ryukyus School of Medicine
  • Nakasone Noboru
    Department of Bacteriology, University of the Ryukyus School of Medicine
  • Yamashiro Tetsu
    Department of Bacteriology, University of the Ryukyus School of Medicine
  • Higa Naomi
    Department of Bacteriology, University of the Ryukyus School of Medicine

抄録

The distribution of Vibrio cholerae O1 pili consisting of 16kDa subunit protein (16K-pili) was examined by Western blotting, using 211 strains from various origins and specific anti-16K-pili sera. The 16kDa protein was detected in all 211 strains. The pili were purified from 3 El Tor and 3 classical strains, and characterized by hemagglutination and inhibition tests. All purified pili were hemagglutinative. However, the hemagglutinating activity of classical pili disappeared after exposure to 5M urea and the agglutination induced by the classical pili was inhibited by D-mannose, alpha-methylmannoside, D-glucose and N-acetylglucosamine. On the contrary, El Tor pili were resistant to these sugars and urea.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ