Purification of <i>Bacillus subtilis</i> Spore Coat Protein by Electrophoretic Elution Procedure and Determination of NH<sub>2</sub>-Terminal Amino Acid Sequences
-
- Abe Akiko
- Faculty of Pharmaceutical Sciences, Setsunan University
-
- Ogawa Setsuko
- Faculty of Pharmaceutical Sciences, Setsunan University
-
- Kohno Takeyuki
- Faculty of Pharmaceutical Sciences, Setsunan University
-
- Watabe Kazuhito
- Faculty of Pharmaceutical Sciences, Setsunan University
抄録
Spore coat protein of Bacillus subtilis was purified by electrophoretic elution procedure. Solubilized coat protein components were separated on SDS-PAGE and the desired protein was recovered from the gel pieces under the optimal condition examined. Two purified polypeptides with molecular weights of about 40kDa were obtained; each of them was in very closed size on SDS-PAGE, both retaining antigenic activity against anti-spore coat protein serum on immunoblot analysis. The N-terminal 23 and 30 amino acid sequences of them were determined, and they were not identical to each other and also not homologous in the sequences of coat proteins previously reported.
収録刊行物
-
- MICROBIOLOGY and IMMUNOLOGY
-
MICROBIOLOGY and IMMUNOLOGY 37 (10), 809-812, 1993
Center For Academic Publications Japan
- Tweet
詳細情報
-
- CRID
- 1573387452895458944
-
- NII論文ID
- 130003483934
-
- ISSN
- 03855600
-
- 本文言語コード
- en
-
- データソース種別
-
- CiNii Articles